Fig. 2: Single-cell RNA sequencing reveals that HMSC is genomically and transcriptionally distinct from ACC.

A Heatmaps show CNVs in HMSC and ACC non-malignant (top) and malignant (bottom) single cells. Columns represent chromosomal regions; red represents amplification, while blue represents deletion. B Uniform manifold approximation and projection (UMAP) shows that primary malignant cells from HMSC cluster separately from those of ACC tumors. All cells are colored by patient. C UMAP shows MYB expression in primary malignant cells from HMSC and ACC tumors. D UMAP shows Kaye ACC score in primary malignant cells from HMSC and ACC tumors. E Bar plots show gene set enrichment analysis (GSEA) for pathways upregulated in HMSC relative to ACC (left panel, blue bars, dashed line represents false discovery rate (FDR) < 0.05) and pathways upregulated in ACC relative to HMSC (right panel, red bars, dashed line represents FDR < 0.05).