Fig. 1: Mutational frequency of RAS genes and other frequently mutated genes in CRC.

A Mutational frequency of the most commonly mutated cancer-related genes in primary CRCs (stage I-II, III, or IV) and metastases of colorectal origin (M). Cases were classified as MSS (left) or MSI/hypermutated (right) based on their MSI status and tumor mutational burden (see “Methods”). B Differences in mutational frequency of Ras genes, analyzed by multivariable logistic regression. Dots indicate the log odds ratio (x-axis). The horizontal bars represent the 95% CI of the log odds ratio, colored by their statistical significance corrected using Tukey’s HSD method (Sig Level). Values to the left of the vertical dotted line indicate higher mutational frequency in the group listed first in the corresponding comparison (y-axis), while values to the right indicate higher mutational frequency in the group listed second. Two multivariable logistic regression analyses are shown: one including all samples and using sample type (primary tumor or metastasis) as a covariate (upper row), and another exclusively comprising primary tumors to allow for the inclusion of cohort as a covariate (lower row).