Fig. 1: Glioblastoma cell lines differ in their circadian profile. | npj Precision Oncology

Fig. 1: Glioblastoma cell lines differ in their circadian profile.

From: Personalized chronotherapy in glioblastoma: integrating circadian profiling and PK–PD modelling to optimize temozolomide timing

Fig. 1: Glioblastoma cell lines differ in their circadian profile.The alternative text for this image may have been generated using AI.

a Circadian profile on the cellular level. GBM cell lines were transduced with luminescent reporters to record BMAL1 or PER2 promotor activity in living cells. The experiment was repeated three times with three biological replicates. Circadian parameters were calculated by Chronostar 3 software. b The circadian profiles of selected core-clock genes were measured by q-RT-PCR and normalized to GAPDH and the mean gene expression. The experiment was performed in three biological replicate and for each biological replicate three technical replicates were measured. Circadian parameters were determined by cosinor analysis. Error bars signify standard deviation. Crossed boxes in the heatmaps indicate no significant rhythmicity (p > 0.05) in the cosinor analysis. One-way ANOVA was used for statistical comparisons.

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