Fig. 5: Gene expression profiling of three patient #0134-derived xenografts using single cell transcriptomics. | npj Precision Oncology

Fig. 5: Gene expression profiling of three patient #0134-derived xenografts using single cell transcriptomics.

From: Venetoclax resistance in preclinical KMT2A-rearranged acute lymphoblastic leukemia models is characterized by high inter- and intra-model heterogeneity

Fig. 5: Gene expression profiling of three patient #0134-derived xenografts using single cell transcriptomics.

PDX227 represents a vehicle-treated control animal while PDX224 and PDX225 continuously received VEN. A Bulk analysis of key BCL-2 pathway genes. B UMAP plot demonstrating the expression profiles of the control (blue) and the VEN-resistant animals (reds). C Distribution of specific clusters across the three samples. Clusters C3, C5, and C6 of sample PDX225 represent the further investigated resistance-specific signatures and are therefore highlighted. D Genes significantly overexpressed in PDX225-specific clusters C3, C5, and C6 were subjected to enrichment analysis and mapped to Reactome pathways to identify significantly regulated mechanisms. E Volcano plot visualizing the top regulated pathways in PDX225 (Clusters 3, 5, and 6). F Boxplots demonstrating the expression of genes allocated to the GO term Cell cycle throughout the sample-specific clusters. G UMAP plot visualizing Reactome pathways associated with significantly upregulated genes in PDX225-specific clusters 3, 5, and 6. Larger dots indicate a higher degree of regulation, and circled dots represent the top-influenced pathways.

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