Fig. 1: LIONESS study design. | npj Precision Oncology

Fig. 1: LIONESS study design.

From: Personalized ctDNA analysis for detection of residual disease and recurrence in surgically treated HNSCC patients

Fig. 1: LIONESS study design.

A Prospective cohort study population flow diagram showing the study population based on available tissue and testing. B Schematic of LIONESS study design. C RaDaR workflow. Tumor tissue from surgical resection was macrodissected and used for WES to identify somatic mutations followed by designing personalized ctDNA assays. Tumor and buffy coat DNA were analyzed using personalized assays to confirm somatic mutations and exclude germline and clonal hematopoiesis of indeterminate potential (CHIP) variants. Plasma and saliva samples were collected pre- and postoperatively, analyzed using RaDaR panels and high-depth sequencing, and ctDNA detection reported per patient. WES, whole exome sequencing; VAF, variant allele frequency. Figure generated with BioRender.com.

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