Fig. 3: Validation of correlations of CD27 gene expression with biological and immune features in an independent cohort of 91 metastatic SKCMs from PRJEB23709.

A Correlation matrix plot shows correlations of CD27 mRNA expression with multiple checkpoint genes. B Box plot showing differences in the absolute proportions of immune cells in the tumor microenvironment between the high and low CD27 mRNA expression groups. C Heatmap displaying the expression of four immune therapy-related signature gene sets, including IFNγ-6, IFNγ expanded immune 18, effector T-cell and IFNγ/effector T-cell. D-G Box plots of gene set enrichment scores of IFNγ-6 (D), IFNγ expanded immune 18 (E), effector T-cell (F) and IFNγ/effector T-cell (G) in high and low CD27 groups of primary melanoma. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.