Fig. 3: The changes in CEC XV reflect the cellular function.
From: A novel single-cell level evaluation method for corneal endothelial cell function
a Immunofluorenscence staining of ZO-1 (green), Tom20 (red), and corresponding XV visualization in normal and conreal endothelial dysfunction mouse models. b Tom20 expression of Col8a2Q455K mouse model and UVA-induced mouse model were significantly reduced (✱P < 0.05, ✱✱✱P < 0.001). c Both markers showed significant negative correlations with XV. d Immunofluorenscence staining of ZO-1 (green), Hsp70 (red), γ-H2AX (red), and corresponding XV visualization in normal and UVA-induced corneal endothelial dysfunction mouse models. e Hsp70 and γ-H2AX exhibited increased fluorescence intensity with rising XV (✱✱P < 0.01, ✱✱✱P < 0.001). f Both markers showed significant positive correlations with XV. g Slit lamp photography, corneal thickness changes, Alizarin red staining of the endothelium, and XV visualization in rabbits at different time points post-cryoinjury. h Mean XV peaked on the first-day post-cryoinjury, gradually decreasing over time until returning to preoperative values. The central region of the corneal endothelium exhibited significant damage and low cell density after cryoinjury; however, regeneration restored endothelial cell density to preoperative levels over time corneal thickness peaked on the first-day post-cryoinjury and gradually decreased to preoperative levels. (bar = 50 µm, n = 3).
