Extended Data Fig. 9: Distribution of candidate methyltransferase ribozyme sequences.
a, Table of natural SMRZ-1 ribozyme candidate sequences 16-40. The sequences are listed by classification and accession numbers. The alignment and activity relative to SMRZ-1 of each sequence and their relative position in the secondary structure in the RNA is shown. b, Methylation activity of sequences 16-40. The histograms show (Mauve, left axis) uncorrected incorporation of radioactivity (cpm) for each sample compared to the background measurement (Bkg) and the internal SMRZ-1 control for each experiment (separated by vertical dashed lines). The axis break from 300-1000 cpm shows the relatively low background signal (mean of 187 cpm ± 34 (n=15)), error bars are the standard deviations from the mean of 3 independent experiments (shown as contrasting dots). The right-hand (light blue) axis is the normalised (%) activity for each sequence relative to the SMRZ-1 control, for comparison between experiments, error bars are the standard deviations from the mean of 3 independent experiments (shown as contrasting dots).
