Extended Data Fig. 7: Functional characterization of MgcC by in vitro assay.
From: Efficient exploration of terpenoid biosynthetic gene clusters in filamentous fungi
a, The expression verification of MgcC by SDS-PAGE. b, In vitro reaction system for functional verification of MgcC. The reaction included 0.5 μM MgcC enzyme, 1 mM Mg2+, 200 μM substrate (70) and 20 mM phosphate buffer (pH 7.4) was performed at 30 °C for 8 h. c, HR-ESI-MS detection of compound 71 produced by MgcC. Compound 71 were detected in the reaction contained MgcC and substrate 70 (i). The reactions, without MgcC (ii) or without substrate(iii), were unable to produce 71. Three independent experiments were repeated with similar results.
