Extended Data Fig. 8: Comparison of HotPETase and HotPETaseLR.
From: Directed evolution of an efficient and thermostable PET depolymerase
(a) Protein melt curves for HotPETase and HotPETase K212N, E213S, Y214S (HotPETaseLR). Melt curve readings were carried out in triplicate. (b) 24 h time-courses, showing the mean total concentration of released MHET and TPA, in reactions at either 40 °C or 65 °C with either HotPETase or HotPETaseLR, using 0.4% cryPET substrate loading (4 g L−1) and 0.29 mg g−1 enzyme loading (0.04 μM). Reactions were performed in pH 9.2, 50 mM Gly-OH buffer, 4% BugBuster, in triplicate; error bars represent the s.d. of the replicate measurements.
