Extended Data Table 1 PET substrate characterization before and after enzyme reactions

From: Directed evolution of an efficient and thermostable PET depolymerase

  1. PET substrates were characterised pre-reaction (no treatment), following incubation in reaction buffer (buffer-only control) or following enzymatic depolymerization (enzyme reaction). Enzymatic reactions were conducted for 48 h with HotPETase at 60 °C, using 0.4% cryPET substrate loading (4 g L−1) and 0.29 mg g−1 enzyme loading (0.04 μM). Reactions were performed in pH 9.2, 50 mM Gly-OH buffer, 4% BugBuster. The negative, buffer-only control was prepared in a similar fashion without the addition of HotPETase. Crystallinity was assessed by DSC and molecular weights by SEC.
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