Extended Data Fig. 2: Nonspecific nicking activity of E.DndFGH. | Nature Catalysis

Extended Data Fig. 2: Nonspecific nicking activity of E.DndFGH.

From: The functional coupling between restriction and DNA phosphorothioate modification systems underlying the DndFGH restriction complex

Extended Data Fig. 2

Run-off sequencing of open circular pUC19 treated with DndFGH or Nt. BspQI. The peaks in the sequencing chromatogram dropped dramatically at the sequence-specific nicking site of Nt.BspQI. In contrast, no such sequencing peak drop was observed when DndFGH-nicked pUC19 DNA was used as a template despite the presence of the 5′-GAAC-3′/5′-GTTC-3′ motif (red underlined).

Back to article page