Extended Data Fig. 5: A schematic model of PT modification-modulated DndFGH defence. | Nature Catalysis

Extended Data Fig. 5: A schematic model of PT modification-modulated DndFGH defence.

From: The functional coupling between restriction and DNA phosphorothioate modification systems underlying the DndFGH restriction complex

Extended Data Fig. 5: A schematic model of PT modification-modulated DndFGH defence.

a, DndA/IscS and DndBCDE proteins act as the modification component to confer PT modification at a subset of 5′-GAAC-3′/5′-GTTC-3′ sites in host DNA, generating 5′-GPSAAC-3′/5′-GPSTTC-3′. b, As the restriction component, the DndFGH complex undergoes conformational changes to generate DNA nicking and translocation activities. Furthermore, PTs in self-DNA suppress the DNA-stimulated ATP hydrolysis activity of the DndFGH complex and consequently impedes its translocation and nicking activities, thus preventing autoimmunity.

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