Extended Data Fig. 6: Self-vs-nonself discrimination of DndFGH in vitro. | Nature Catalysis

Extended Data Fig. 6: Self-vs-nonself discrimination of DndFGH in vitro.

From: The functional coupling between restriction and DNA phosphorothioate modification systems underlying the DndFGH restriction complex

Extended Data Fig. 6: Self-vs-nonself discrimination of DndFGH in vitro.

When PT-modified (PT+) pUC19 and unmodified (PT−) pACYC184 DNA were mixed at a 1:1 ratio and subjected to the nuclease digestion of E.DndFGH, PT+ pUC19 remained intact, whereas pACYC184 was specifically damaged. Without PT modification, PT− pUC19 DNA is also susceptible to E.DndFGH. Results are representative of three independent experiments.

Source data

Back to article page