Extended Data Fig. 7: Cryo-EM workflow of Tetrahymena intron G264A mutation self-splicing from transcription, related to Fig. 2. | Nature Catalysis

Extended Data Fig. 7: Cryo-EM workflow of Tetrahymena intron G264A mutation self-splicing from transcription, related to Fig. 2.

From: Cryo-EM reveals dynamics of Tetrahymena group I intron self-splicing

Extended Data Fig. 7: Cryo-EM workflow of Tetrahymena intron G264A mutation self-splicing from transcription, related to Fig. 2.

(a) Denatured gel analysis of transcription mixture showed all intermediate and final self-spliced products, representative of three independent experiments. (b) Cryo-EM single-particle workflow yielded three conformations corresponding to relaxed and intermediate pre-TetG264A-S1, and docked TetG264A-S1 states, coloured according to local resolution maps with angular distribution and FSC curves indicating resolutions according to the 0.143 cutoff.

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