Extended Data Fig. 1: Cryo-EM workflow of Tetrahymena intron self-splicing from transcription, related to Fig. 1. | Nature Catalysis

Extended Data Fig. 1: Cryo-EM workflow of Tetrahymena intron self-splicing from transcription, related to Fig. 1.

From: Cryo-EM reveals dynamics of Tetrahymena group I intron self-splicing

Extended Data Fig. 1

(a) Denatured gel analysis of transcription mixture showed all intermediate and final self-spliced products, representative of three independent experiments. (b) Cryo-EM single-particle workflow yielded three conformations corresponding to Tet-S2a, Tet-S2b and pre-Tet-C states, coloured according to local resolution maps with angular distribution and FSC curves indicating resolutions according to the 0.143 cutoff.

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