Fig. 6: Application in synthesis. | Nature Catalysis

Fig. 6: Application in synthesis.

From: Engineered cytochrome P450 for direct arylalkene-to-ketone oxidation via highly reactive carbocation intermediates

Fig. 6

a, Substrate scope of the KS reaction. Reactions were carried out using 0.625 µM KS, 5 mM of the corresponding substrate and 5 mM NADH cofactor. TTN values were determined after 2 h reaction time. aMaximum TTN was determined after 48 h biotransformation combined with a cofactor regeneration system. b, KS application on a preparative scale for the synthesis of ketones as well as in the formal asymmetric hydration and hydroamination of internal alkenes. See Supplementary Figs. 16 and 17 for more details. e.r., enantiomeric ratio; GDH, glucose dehydrogenase; PAR, phenylacetaldehyde reductase; LBv-ADH, alcohol dehydrogenase from Lactobacillus brevis; IRED, imine reductase pIR-23 (Cystobacter ferrugineus (CF)IRED).

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