Extended Data Fig. 8: In vitro assays of the SoBcmB mutants using compound 2 as the substrate.
a, Enzymatic activities of SoBcmB and its mutants. Reactions were performed at 37 °C for 1 h using compound 2 as the substrate in 50 μl 50 mM Tris–HCl (pH 7.5) buffer containing 50 μg SoBcmB or one of its mutants. Different colour columns represent different products. Bars represent mean conversion yield averaged over three reactions and error bars indicate standard deviation of three independent replicates. Asterisks represent enzyme activity not detected. b, Structures of products 2a, 2d, and 2f–2i. See Extended Data Fig. 9a and Supplementary Fig. 17 for HPLC chromatograms.
