Fig. 1: The THETA cycle and its realization.
From: Construction and modular implementation of the THETA cycle for synthetic CO2 fixation
a, The overall strategy used in this work to establish the THETA cycle. The THETA cycle was designed in three modules, constructed in vitro, optimized through rational approaches and machine learning, before its three modules were implemented in E. coli. b, The scheme of the THETA cycle. Inner circle: simplified scheme of the THETA cycle, consisting of three pathway modules. Outer circle: detailed scheme of the THETA cycle. The conditions for ΔrG′m are at 25 °C, 1 bar pressure, pH 7.0, pMg 3, ionic strength 0.25 M and 1 mM for all reactants. The calculation details of ΔrG′m can be found at ref. 47. For enzyme abbreviations, see Supplementary Table 2. ML, machine learning.
