Fig. 3: PHIP effects observed for water.
a, An overlay of single-scan 1H spectra acquired after supplying p-H2 (magenta, 99% enrichment) or n-H2 (black) to a sample containing 12 µM jHmd, 120 µM 13CH2=H4MPT and 10 mM formaldehyde-13C in D2O buffer (pD 7.0, 1 mM EDTA and 120 mM potassium phosphate). Experiments were performed at 327 K and 7.1 T, using a 1H-PHIP experiment with signal saturation (sat.) before acquisition (Supplementary Fig. 2). The insert shows the water signals overlaid that were obtained in three repetitions of the experiment with p-H2 or with n-H2. b, Time evolution of the HD (orange) and HDO signal integrals after stopping the signal saturation. The graph displays the difference between the integrals obtained in the p-H2 and the n-H2 experiments. The average and the s.d. over the three repetitions are shown.
