Fig. 5: Transfer of hyperpolarization from (C14a)Hpro-R to C14a.
a, Scheme of polarization transfer from (C14a)Hpro-R to 13C14a using insensitive nuclei enhancement by polarization transfer (INEPT) (Supplementary Fig. 4). b, Refocused INEPT experiment with 1H decoupling during acquisition. c, INEPT experiment without refocusing and decoupling. Overlays of experiments using p-H2 (magenta, 99% enrichment) or n-H2 (black) are shown. Data collected using Nuclear Overhauser Effect (NOE)-driven hyperpolarization transfer to 13C14a (Supplementary Fig. 5) are shown in Supplementary Fig. 16. High-concentration samples were used (sample conditions as stated in Fig. 3 caption, and for a and b only, 2 mM formaldehyde-13C was used), experiments were performed at 327 K and signals were accumulated over 512 scans (512 treatments with p-H2 or n-H2).
