Fig. 1: Schematic representation of the rimocidin PKS-TR platform in Streptomyces.
From: A polyketide-based biosynthetic platform for diols, amino alcohols and hydroxy acids
a, Using glucose (and l-valine) as the carbon source, engineered rimocidin PKS can load three CoA starter units and three extender units. Incorporation of a PKS TR led to the production of diverse products via programmed post-PKS modification by alcohol dehydrogenases (ADHs), aldehyde dehydrogenases (ALDHs) or transaminases (TAs). The dashed lines in the precursor pathways indicate multiple steps. TCA, tricarboxylic acid; α-KG, α-ketoglutarate; KSS, non-canonical KS in rimocidin loading module; TD, terminal domain; CoL, CoA ligase. In the substituent codes R1a/b/c and R2a/b/c, the notation a/b/c denotes different chemical groups that are accepted as substrates for the specified reactions. b, Summary of all the products elaborated in this study. Molecules highlighted in red are industrially valuable and underlined products were microbially synthesized here as featured products. e.e., enantiomeric excess. Created with BioRender.com.
