Fig. 5: Expanding the RimPKS-TR biosynthetic platform for medium- and branched-chain diol and carboxylic acid production.
From: A polyketide-based biosynthetic platform for diols, amino alcohols and hydroxy acids
a, Schematic showing PKS-TR engineering via AT exchange and post-PKS modification. In the substituent codes R1a/b/c and R2a/b, the notation a/b/c denotes different chemical groups that are accepted as substrates for the specified reactions. b, Bioproduction of 2-methyl-1,3-diols in S. albus RimM0M1(Rim/PimM7 AT)-TRs. c, Bioproduction of 2-ethyl-3-hydroxyhexanoic acid in engineered S. albus RimM0M1(RimM13 AT)-TR2/TEs. TE1, DEBS TE; TE2, pikromycin TE; TE3, Rim TE. d, Bioproduction of insect repellent 2-E-1,3-HDO in S. albus RimM0M1(M13 AT)-TR2 + ADH + CAR. All data were collected after cultivation for 7 d in R5 + 2% glucose + 15 mM l-valine. The data are presented as mean values; the error bars indicate the s.d. of three biological replicates (n = 3).
