Extended Data Fig. 4: Image processing workflow for Cryo-EM data.

A) Cryo-EM data processing workflow using CryoSPARC v4.2.1. The 3D maps were displayed using UCSF ChimeraX66,73,74. The false color scale indicates the local resolution B) Orientation distribution of particles that produced the final electron density map in (A). C) Fourier Shell Correlation global resolution plot at FCS = 0.143 generated by CryoSPARC for final electron density map in (A).