Extended Data Fig. 2: MAR Characterization. | Nature Catalysis

Extended Data Fig. 2: MAR Characterization.

From: Architecture, catalysis and regulation of methylthio-alkane reductase for bacterial sulfur acquisition from volatile organic compounds

Extended Data Fig. 2: MAR Characterization.

A-B) Maldi-TOF mass spectrometry of isolated MarH and MarDK + A3441 protein from DEAE chromatography (Fig. 2). ADPR; ADP-ribose post-translational modification. Experiments conducted for n = 2 samples with same results. C) SDS-page gel electrophoresis of MarH from size exclusion purification (Fig. 2) under varying reducing conditions. D-E) AlphaFold predicted structure (UniProt: Q2RNRO) and sequence of the A3441 protein colored by predicted local density difference test. A3441 contains a predicted winged helix domain and a C-terminal helical domain homologous to other winged-helix DNA- and protein-binding dimeric regulatory proteins. In addition, it contains a glycine-rich (31%) N-terminal domain. F) Gas chromatography analysis for Fig. 3f of MarHDK activity in the presence of CO showing that CO is not reduced to methane or other hydrocarbons by MAR.

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