Table 1 MAR purification and activity

From: Architecture, catalysis and regulation of methylthio-alkane reductase for bacterial sulfur acquisition from volatile organic compounds

Sample

Purification step

Total MAR Activity

(mU = nmol min−1)

Total protein

(mg)

Specific MAR activity

(nmol min−1 g−1)

Total cellular

protein (%)

R. rubrum native MAR system

Whole cell

12.6

1,600

7.9 ± 2.2

100

Soluble fraction

12.9

1,332

9.7 ± 2.9

83

Insoluble fraction

–

–

<1

–

DEAE eluate

10.2

5.1

2,000 ± 354

0.3

DEAE flowthrough

–

–

0

–

R. rubrum MAR overproduction system

Whole cell

148

1,760

84 ± 9

100

Soluble fraction

145

1,440

101 ± 15

82

Insoluble fraction

–

–

<1

–

Ni-NTA eluate

575

25

23,000 ± 3,105

1.4

DEAE eluate

699

20

34,947 ± 4,488

1.1

  1. Activity, enrichment and percentage of total cellular protein by mass of MAR purified natively and from plasmid-based overproduction in R. rubrum. Averages and standard deviation error bars are for n = 3 independent experiments. DEAE, diethylaminoethanol anion exchange.
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