Fig. 4

Cholesterogenesis affected by Ad4BP/SF-1 knockdown. a The activity of de novo cholesterogenesis was determined by quantification of ¹⁴C-cholesterol synthesized from ¹⁴C-acetate. The amounts of ¹⁴C-cholesterol in Ad4BP/SF-1 knockdown and control Y-1 cells are shown. Average values and SDs are indicated. *p < 0.01. n = 4. b The amounts of cholesterol, intermediate products (lanosterol, lathosterol, desmosterol, and 7-DHC), and esterified cholesterols (Chol-M and Chol-A) were determined in Ad4BP/SF-1 knockdown and control Y-1 cells. Average values and SDs are indicated. *p < 0.05, **p < 0.01. n = 3. 7-DHC 7-dehydrocholesterol, Chol-M cholesteryl myristate, Chol-A cholesteryl arachidonate. Average values and SDs are indicated