Fig. 3 | Communications Biology

Fig. 3

From: Multiple claudin–claudin cis interfaces are required for tight junction strand formation and inherent flexibility

Fig. 3

Mutations of residue M68 do not disrupt tight junction strand formation. a Top and side view of the X-1 dimer of mCldn15 in cartoon representation, and close view of the indicated box highlighting the previously described ECH/ECL1 and TM3/ECL2 interface region; the side chains of residues M68, F146, and F147 are shown as sticks. b Fluorescence confocal images of COS7 cells expressing mCldn15-GFP WT, M68A, and M68E (green) forming tight junctions (arrows) at sites of cell–cell contact. Cellular actin was counterstained with Alexa-405 phalloidin (shown in magenta). c Freeze-fracture TEM of HEK293T expressing mCldn15-GFP WT, M68A, and M68E showing the characteristic tight junction strand morphology. Scale bars = 5 µm for confocal images and 200 nm for freeze-fracture TEM images

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