Fig. 1 | Communications Biology

Fig. 1

From: A universal bioluminescence resonance energy transfer sensor design enables high-sensitivity screening of GPCR activation dynamics

Fig. 1

Evaluation of intramolecular FRET and BRET α2A-adrenergic receptor biosensors. a Schematic of the biosensor. b Emission peaks of chromophores. c Workflow. d FRET emission spectra of the CFP-label alone or with fluorescent acceptors (each N = 1). e FRET changes (%) induced by norepinephrine normalized for buffer (YFP: N = 7, cpVenus173: N = 4, diAcFAM: N = 6, Oregon Green: N = 5, R110 Direct: N = 3,TMR Direct: N = 6, NanoBRET 618: N = 6, 505-star: N = 5, TMR-star: N = 5, 647SiR: N = 4). f BRET emission spectra of the NanoLuc-label alone or with fluorescent acceptors (each N = 1). g BRET changes (%) induced by norepinephrine normalized for buffer (cpVenus173: N = 7, TagRFP: N = 4, mCherry: N = 4, diAcFAM: N = 3, Oregon Green: N = 3, R110 Direct: N = 3, TMR Direct: N = 3, NanoBRET 618: N = 7, 505-star: N = 3, TMR-star: N = 5, 647SiR: N = 5). Data in e and g show box and whisker plots. Difference was analyzed by two-way ANOVA followed by Bonferroni post hoc test. *p ≤ 0.05 vs. buffer

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