Fig. 3
From: De novo genome assembly of Oryza granulata reveals rapid genome expansion and adaptive evolution
FISH mapping of centromeric and pericentromeric probes on pachytene chromosomes of O. granulata and phenotypic characterization of its pachytene chromosomes. a Immunodetection of rabbit antibody against rice CENH3 on pachytene chromosomes of O. granulata. b FISH identification of precipitated DNA isolated by ChIP using rice anti-CENH3 antibodies. c Dual color FISH of GCS1 cloned from ChIP DNAs and RIRE2. GCS1 was labeled with digoxigenin-dUTP (magenta), while RIRE2 was labeled with biotin-dUTP (cyan). d Immuno-FISH detection of RIRE2. Rice anti-CENH3 antibodies were used to label centromeres. e Pachytene chromosome morphology of O. granulata. Arrows indicate the 12 centromere constrictions on each pachytene chromosome of O. granulata. f Pachytene chromosome morphology of O. sativa. Chromosomes were counterstained with DAPI. Scale bars = 5 μm
