Fig. 7
Larvae resume feeding on alleviation of hyperglycemia. a w1118; Dcg-Gal4 control and flyabetic larvae (≥75 larvae each) were cultured in 5% sucrose solution for 3 h, after which glucose and trehalose were measured in feeding and non-feeding flyabetic larvae, and compared to controls. b Feeding and non-feeding flyabetic larvae imaged at 3 h. c Relative glucose (left) and trehalose (right) levels, compared to feeding controls, were measured in feeding and non-feeding larvae. d Relative glucose (left) and trehalose (right) levels, and two-dye sucrose feeding were compared in control and Ck1alphaRNAi larvae chronically cultured in the presence or absence of trehalase inhibitor drug. e Glucose levels were measured and two-dye sucrose feeding observed in controls and Ck1alphaRNAi larvae cultured in the presence or absence of Phlorizin. n ≥ 3 each (≥10 larvae per glucose/trehalose replicate). ≥30 larvae per feeding sample. Error bars indicate s.d. Statistical significance was assessed by two-tailed Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001
