Fig. 1
From: In situ serial crystallography for rapid de novo membrane protein structure determination
Overview of the IMISX-EP workflow for high-throughput in situ de novo phasing of in meso-grown microcrystals of membrane proteins. a IMISX crystallization, well preparation, and post-crystallization treatment, snap-cooling, and storage. b Automated grid scanning and serial data collection on hundreds of microcrystals in situ with a fast frame-rate detector. c Selection of isomorphous data sets based on intensity correlation coefficients (red line), analysis of crystal orientations (inset), and phase determination
