Fig. 3
From: In situ serial crystallography for rapid de novo membrane protein structure determination
Structures of membrane proteins solved by IMISX-EP and the crystals used for data collection. Photographic images of crystals in IMISX wells held in the cryo-stream were recorded with an in-line microscope at beamline X06SA-PXI. a Se-PepTSt, 2.7 Å, 89 crystals, two wells, Se-SAD, 18 Se. b Se/S-LspA, 3.0 Å, 497 crystals, 32 wells, Se-SAD, 12 Se. c Hg-BacA IMISX-soaking, 3.0 Å, 360 crystals, eight wells, Hg-SAD, 1 Hg. d Hg-BacA co-crystallization, 2.6 Å, 55 crystals, two wells, Hg-SAD, 2 Hg. e W-PgpB, 1.8 Å, one crystal, one well, W-SAD, 1 W. f S-PepTSt, 2.7 Å, 1595 crystals, 59 wells, S-SAD, 13 S. Structures are shown in ribbon representation with anomalous sub-structures depicted as spheres and SHELXE anomalous Fourier maps contoured at 5 σ. The SHELXE anomalous Fourier map was calculated using the observed anomalous difference (|FA|) and the phases of the sub-structure determined using SHELXD. The black arrows point to representative crystals in each well. The white scale bar corresponds to 20 μm
