Fig. 3

Meiocytes null for MZIP2 were arrested at a zygotene-like stage. a SYCP1 staining showing the zygotene-like stage of MZIP2-deleted spermatocytes. Z-like zygonema-like. White arrow indicates XY body. The regions bordered with dashed lines were enlarged on the right. Scale bar, 10 μm. b Meiotic progression of WT and Mzip2^−/− spermatocytes throughout meiotic prophase I. Numbers of spermatocytes analyzed (n) were indicated. Z zygonema, P pachynema, D diplonema, Z* zygotene-like. Error bars indicates S.E.M. c Quantification of SYCP1 stretches in WT pachynema spermatocytes and Mzip2^−/− zygonema-like spermatocytes. Numbers of spermatocytes analyzed (n) were indicated. Error bars indicated S.E.M. d HORMAD1 staining showing the zygotene-like stage of MZIP2-deleted spermatocytes. Scale bar, 10 μm. e Meiotic progression of oocytes derived from WT and Mzip2^−/− females at E17.5. Numbers of spermatocytes analyzed (n) were indicated. f SYCP1 staining on nuclear surface spreads of oocytes derived from WT and Mzip2^−/− females at E17.5. Scale bars, 10 μm. g Staining of a telomeric marker (TRF1) and a centromeric marker (CREST) on nuclear surface spreads of WT and Mzip2^−/− spermatocytes. White arrow indicates XY body. Scale bars, 10 μm. h–i Quantification of TRF1 and CREST foci on nuclear surface spreads of WT spermatocytes at a pachytene stage or MZIP2-deleted spermatocytes arrested at a zygotene-like stage