Fig. 4

MZIP2-null meiocytes failed to repair meiotic DSBs. a γH2AX (green) staining on nuclear surface spreads of WT and Mzip2^−/− spermatocytes and primordial germ cells at indicated stages. White arrow indicates XY body. Scale bar, 10 μm. b Nuclear surface spreads of WT and Mzip2^−/− spermatocytes were stained with the early recombination marker, RAD51. White arrow indicates XY body. The regions bordered with dashed lines were enlarged on the right. Scale bar, 10 μm. c Quantification of RAD51 foci detected in WT and Mzip2^−/− spermatocytes or oocytes at indicated stages. d, e RPA1 was stained in WT and Mzip2^−/− spermatocytes at different stages (d), and the quantification of which was shown in e. White arrow indicates XY body. Scale bars, 10 μm