Fig. 5

Defective meiotic recombination and crossing-over in MZIP2-deleted meiocytes. a–c Staining of TEX11 on nuclear surface spreads prepared with WT and Mzip2^−/− testes (a) or ovaries (b). The quantification of TEX11 foci was shown in c. Scale bars, 10 μm. d–f Staining for spermatocytes (d) and oocytes (e), and quantification (f) of MSH4 on the nuclear surface spreads. Scale bars, 10 μm. g–i MLH1 was stained to indicate late recombination intermediates in WT and MZIP2-deleted spermatocytes (g) or oocytes (h). The quantification of MLH1 foci was shown in i. Scale bars, 10 μm