Fig. 1
The picalm5a picalm5b double mutation leads to reduced male fertility. a and b Shoots (a) and inflorescences (b) of six-week-old wild-type (WT), picalm5a, picalm5b, picalm5a picalm5b, PICALM5a-GFP in picalm5a picalm5b, and PICALM5b-GFP in picalm5a picalm5b plants. Scale bars = 10 cm (a) and 1 cm (b). c Representative cleared siliques of self-pollinated WT and picalm5a picalm5b plants. Scale bar = 1 mm. d Quantification of seeds per silique for wild-type, picalm5a, picalm5b, picalm5a picalm5b, PICALM5a-GFP in picalm5a picalm5b, and PICALM5b-GFP in picalm5a picalm5b plants (n = 30). Bars represent the means and the asterisk indicates a significant difference from the wild-type plant according to Welch’s t test (p = 3.04 × 10−28). e Representative cleared siliques obtained by cross pollination between parents with the indicated genotypes. 5a 5b represents the picalm5a picalm5b double mutant. Scale bar = 1 mm. f Quantification of the seeds per silique for cross-pollinated siliques (n = 10 siliques for each combination). 5a 5b represents the picalm5a picalm5b double mutant. Bars represent the means and asterisks indicate a significant difference from the value for crosses between male and female wild-type plants according to Welch’s t test (p = 1.80 × 10-8 for cross between male 5a 5b and female wild-type plants and 1.72 × 10-9 for male and female 5a 5b plants)
