Fig. 2
PICALM5a and PICALM5b are essential for sustained pollen tube growth. a Aniline blue staining of pistils from wild-type, picalm5a, picalm5b, picalm5a picalm5b, PICALM5a-GFP in picalm5a picalm5b, and PICALM5b-GFP in picalm5a picalm5b plants. Pistils were harvested 12 h after hand pollination. The white arrows indicate the tips of the longest pollen tubes. Scale bar = 500 μm. b Quantification of lengths of the longest pollen tubes in pistils 12 h after pollination (n = 10 pistils). Bars represent means and the asterisk indicates a significant difference from the result of the wild type according to Welch’s t test (p = 1.64 × 10-5). c Wild-type (WT) and picalm5a picalm5b pollen tubes grown in vitro. Arrow heads and an arrow indicate knotted structure and burst tip of picalm5a picalm5b pollen tubes. Scale bar = 100 μm. d Quantification of wild-type, picalm5a, picalm5b, picalm5a picalm5b, PICALM5a-GFP in picalm5a picalm5b, and PICALM5b-GFP in picalm5a picalm5b pollen tube lengths after 5 h of incubation (n = 60). Bars represent means and the asterisk indicates a significant difference from the WT pollen tubes according to Welch’s t test (p = 3.84 × 10-19). e WT and picalm5a picalm5b pollen tubes grown semi-in vivo 2 and 3 h after pollination. Magnified pollen tube tips are also shown for pollen tube tips 3 h after pollination. Arrowheads indicate burst pollen tubes. Scale bars = 100 μm and 20 μm for low and high magnification images, respectively. HAP hours after pollination
