Fig. 3
Localization of PICALM5a and PICALM5b in growing pollen tubes. a–d GUS staining of pistils (a, c) and anthers (b, d) from PICALM5apro:GUS (a, b) and PICALM5bpro:GUS (c, d) plants. Scale bars = 500 μm (a, c) and 100 μm (b, d). e, f Subcellular localization of PICALM5a-GFP (e) and PICALM5b-GFP (f) in pollen tubes from the picalm5a and picalm5b mutants germinated in vitro. Scale bar = 10 μm. The data are representatives of more than ten pollen tubes observed for each transgenic line. g Subcellular localizations of CLC1-GFP and PICALM5a-mRFP in a wild-type pollen tube germinated in vitro. Scale bar = 5 μm. The data are representatives of more than 12 pollen tubes observed. h Fluorescence intensity profiles for the white box shown in g. i, j Subcellular localizations of CLC1-GFP in a growing pollen tube from a wild-type (i) plant and a picalm5a picalm5b (j) plant germinated in vitro. Scale bar = 10 μm. The representative data of more than ten pollen tubes observed for each transgenic line are presented
