Fig. 4

PICALM5a and PICALM5b are required for the correct localization of ANXUR receptor-like kinases. a Subcellular localizations of ANX1-GFP and ANX2-GFP in growing pollen tubes of wild-type, picalm5a, picalm5b, and picalm5a picalm5b plants germinated in vitro. Scale bar = 10 μm. The data are representatives of more than seven pollen tubes observed for each transgenic line. b Subcellular localizations of ANX2-GFP in in vitro-germinated growing pollen tubes of transgenic plants harboring a single copy of ANX2-GFP expressed with or without PICALM5a-mRFP on the picalm5a picalm5b background. Scale bar = 10 μm. The data are representatives of six (with PICALM5a-mRFP) or four (without PICALM5a-mRFP) pollen tubes observed, respectively