Fig. 5
PICALM5a and PICALM5b are not required for the correct localization of SYP1 Qa-SNAREs and PRK6 receptor-like kinase. a Subcellular localizations of GFP-SYP124, GFP-SYP125, and GFP-SYP131 in growing pollen tubes of wild-type (WT) and picalm5a picalm5b plants germinated in vitro. Scale bar = 10 μm. The data are representatives of more than ten pollen tubes observed for each transgenic line. b Subcellular localizations of PRK6-mClover in growing pollen tubes of WT and picalm5a picalm5b plants germinated in vitro. Bright-field (BF) images are also shown. Scale bar = 10 μm. The data are representatives of five pollen tubes observed for each transgenic line. c WT and picalm5a picalm5b pollen tubes just after (0 min) and 5 min after application of AtLURE1.2-containing gelatin beads. Asterisks indicate AtLURE1.2-containing gelatin beads. Scale bar = 50 μm. Both wild-type and picalm5a picalm5b pollen tubes were attracted to AtLURE1.2
