Fig. 1

Overall structure of NTMT2. a Ribbon diagram of NTMT2 in ternary complex with SAH and a SPKRIA peptide derived from N-terminal RCC1. b Structural alignment of NTMT1 (PDB: 5E1B) and NTMT2. c Isothermal titration calorimetry measurement of the interaction between NTMT2 and the SPKRIA peptide. d Cross-section view of the SAH-SPKRIA-binding pocket. The electrostatic potential surface of NTMT2 plotted at ±5 kT/e (red, negative; blue, positive). e Close-up views of the S-P-K motif recognition by NTMT2 and NTMT1 (PDB: 5E1B). Residues of NTMT2 and NTMT1 involved in the interactions are labeled and shown in green and cyan, respectively. Hydrogen bonds are indicated as dashed lines, and water molecule is shown as red sphere