Table 1 Data collection and refinement statistics
From: An asparagine/glycine switch governs product specificity of human N-terminal methyltransferase NTMT2
NTMT2-SAM | NTMT2-SAH-SPKRIA | |
|---|---|---|
Data collection | ||
Space group | P 21 21 2 | P 43 |
Cell dimensions | ||
a, b, c (Å) | 45.59, 132.96, 42.29 | 44.36, 44.36, 262.06 |
α, β, γ (°) | 90.00, 90.00, 90.00 | 90.00, 90.00, 90.00 |
Resolution (Å) | 45.60-2.00 (2.05–2.00) | 31.14–1.20 (1.22–1.20) |
Rsym or Rmerge | 0.086 (0.700) | 0.074 (0.973) |
I/σI | 10.2 (1.7) | 11.0 (2.0) |
Completeness (%) | 94.4 (91.7) | 96.0 (93.2) |
Redundancy | 2.6 (2.6) | 6.1 (6.6) |
Refinement | ||
Resolution (Å) | 18.98–2.00 | 31.10–1.20 |
No. of reflections | 16,002 | 145,394 |
Rwork/Rfree | 0.208/0.250 | 0.154/0.178 |
No. of atoms | 1780 | 4160 |
Protein | 1691 | 3690 |
Ligand/ion | – | 72 |
Water | 63 | 314 |
B-factors | 27.4 | 17.0 |
Protein | 27.3 | 16.1 |
Ligand/ion | – | 17.6 |
Water | 28.9 | 25.7 |
R.m.s. deviations | ||
Bond lengths (Å) | 0.014 | 0.02 |
Bond angles (°) | 1.7 | 1.9 |
