Fig. 2 | Communications Biology

Fig. 2

From: Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin

Fig. 2

Binding affinities within ACA8-CaM complex investigated by fluorescence anisotropy. a Binding of fluorescein-labeled CaM to full-length ACA8 (Kd = 18 nM). b Binding of fluorescein-labeled regulatory domain (RD) to ACA8core (Kd = 1.7 μM). c Addition of CaM to a preformed complex containing ACA8core and labeled RD. Decrease in anisotropy indicates displacement of RD from ACA8core through CaM-binding to RD. All experiments were performed using protein reconstituted in nanodiscs

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