Table 2 SANS parameters
From: Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin
ACA8-sND (apo) | ACA8-sND + dCaM | ACA8-sND + CaM | |
|---|---|---|---|
SANS | SANS | SANS | |
(SASDES4) | (SASDET4) | (SASDEU4) | |
Data collection parameters | |||
Instrument | SANS-1 (MLZ, Munich) | SANS-1 (MLZ, Munich) | SANS-1 (MLZ, Munich) |
Wavelength (Å) | 5.0 | 5.0 | 5.0 |
q range (nm−1) | 0.12–2.4 | 0.12–2.4 | 0.12–2.4 |
Exposure time (s) | 7200 | 7200 | 7200 |
Concentration (mg/ml) | 3.5 | 3.2 | 3.0 |
Temperature (K) | 283 | 283 | 283 |
Structural parameters | |||
I(0) (cm−1) [from p(r)] | 0.11 | 0.1 | 0.15 |
Rg (Å) [from p(r)] | 42.5 | 45.8 | 52.9 |
I(0) (cm−1) (from Guinier) | 0.11 | 0.092 | 0.1 |
Rg (Å) (from Guinier) | 40 | 42.8 | 50 |
Dmax (Å) | 130 | 150 | 180 |
Porod volume estimate (Å3) | 202000 | 217000 | 297000 |
Molecular-mass determination | |||
Partial specific volume (cm3 g−1) | 0.744 | 0.744 | 0.739 |
Contrast (Δp x 1010 cm−2) | −3.320 | −3.320 | −3.299 |
Molecular mass Mr (Da) [from Porod volume (Vp/1.6)] | 126250 | 135625 | 185625 |
Calculated Mr from sequence (Da) | 118000 | 118000 | 150000 |
Software employed | |||
Primary data reduction | BerSANS | BerSANS | BerSANS |
Data processing | PRIMUS/Qt | PRIMUS/Qt | PRIMUS/Qt |
Rigid-body modeling | EOM | ||
Computation of model intensities | CRYSON | CRYSON | CRYSON |
3D graphics representations | PyMOL | PyMOL | PyMOL |
