Fig. 2
Optical redox ratio of the wound edge changes over time. a In vivo redox ratio maps of FAD/(NADH+FAD) were generated from the normalized fluorescence intensities. The representative optical sections from the z-stacks were acquired at the wound edge, 125 µm from the surface of the tissue (scale bar = 100 µm). An average optical redox ratio at the wound edge was calculated from the pixels contained within the epithelium (white dashed lines). b Both diabetic and control mice experienced an initial decrease in the epithelial redox ratio from day 1 to days 3 and 5 (p < 0.0002), followed by a gradual increase in redox ratio until day 10. By day 10, the diabetic group had a lower redox ratio than the controls (p = 0.0385). c Wound size in the diabetic group displayed characteristic delays in healing (p < 0.0001). Boxes correspond to the first and third quartiles, and whiskers extend to the full range of the data
