Fig. 2
From: Identification of genes required for eye development by high-throughput screening of mouse knockouts
Corneal abnormalities in Fam20a, Col6a2, and Nadsyn1 knockout mice. Biomicroscopy (a) of Fam20a knockout mice revealed polygonal opacities with indistinct edges and interweaving clear spaces in the corneal stroma (arrow), which were also apparent (arrow) on retro-illumination (b), scale bars = 500 µm. Histology (c) shows superficial stromal mineralization (arrow, scale bar = 50 µm). Corneal vascularization (arrow) and chronic superficial keratitis (arrowheads) were observed in Nadsyn1 knockout mice (d, scale bar = 500 µm), and red blood cells (arrow) are shown in the lumen of neovascular vessels on histology (e, scale bar = 20 µm). Mice lacking Col6a2 had subtle corneal stromal opacities seen on slit-lamp examination, which electron microscopy revealed to be a basket weaving appearance (arrowheads) of the corneal stroma (f) that was not seen in wild type (WT) controls (g), scale bars = 5 µm
