Fig. 2 | Communications Biology

Fig. 2

From: Acute tobacco smoke exposure exacerbates the inflammatory response to corneal wounds in mice via the sympathetic nervous system

Fig. 2The alternative text for this image may have been generated using AI.

ATSE enhances SNS signaling activity and mobilization of neutrophils and γδ T cells into the circulation and corneal limbus. a ELISA-measured plasma epinephrine concentration after corneal abrasion in RA, ATSE, and ATSE + 6-OHDA treatments (two-way AVOVA, interaction p < 0.0001, Sidak’s multiple comparisons test, n = 4). b ELISA-measured plasma norepinephrine concentration after corneal abrasion in RA, ATSE, and ATSE + 6-OHDA treatments (two-way AVOVA, interaction p < 0.0001, Sidak’s multiple comparisons test, n = 4). c Representative flow cytometric quantification of CD11b+Ly6G+ neutrophils in peripheral blood of mice without corneal abrasion 1 h after RA, ATSE-only, and 6-OHDA + ATSE treatments. d Cumulative neutrophil (CD11b+Ly6G+) percentage among total leukocytes in peripheral blood 1 h after ATSE (one-way ANOVA, Tukey's post hoc, p < 0.0001 for RA vs. ATSE and ATSE vs. ATSE + 6-OHDA, n = 6 mice per group). e Representative flow cytometric quantification of γδ T cells in peripheral blood of mice without corneal abrasion 1 h after RA, ATSE-only, and 6-OHDA + ATSE. f Cumulative percentage of γδ T cells (GL3+) among total leukocytes in peripheral blood 1 h after ATSE (one-way ANOVA, Tukey's post hoc, p < 0.0001 for RA vs. ATSE and ATSE vs. ATSE + 6-OHDA, n = 6 mice per group). g Representative Ly6G+ neutrophil (labeled with FITC-Gr1: green) recruitment around limbal vessels (epithelial cell marker CD31: red) of mouse without corneal abrasion 1 h after RA, ATSE-only, and 6-OHDA + ATSE treatments; ×40, scale bars 40μm. h Quantification of neutrophil recruitment to corneal limbus 1 h after RA, ATSE-only, and 6-OHDA + ATSE treatments (one-way ANOVA, Tukey's post hoc test, p < 0.0001 for RA vs. ATSE and ATSE vs. ATSE + 6-OHDA, n = 5). i Representative GL3+ γδ T cell (anti-GL3: red) accumulation in limbus 1 h after RA, ATSE-only, and 6-OHDA + ATSE treatments; ×40, scale bars 40μm. j Quantification of γδ T cell recruitment to corneal limbus 1 h after RA, ATSE-only, and 6-OHDA + ATSE treatments (one-way ANOVA, Tukey's post hoc, p < 0.0001 for RA vs. ATSE, ATSE vs. ATSE + 6-OHDA, n = 5); *, #p < 0.05; **, ##p < 0.01; ***, ###p < 0.001, comparing groups as indicated

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