Fig. 6

Effects of IL-6 neutralization (using IL-6 antibody mAb IL-6) on ATSE-induced wound healing delay and exacerbated inflammation. a Relative expression of IL-6 using mRNA isolated from whole wounded corneas of RA, ATSE, and ATSE + 6-OHDA mice at 6, 12, 18, and 24 h after corneal abrasion (one-way ANOVA, Tukey's post hoc test, RA vs. ATSE, *p < 0.05, **p < 0.01, ***p < 0.001; RA vs. ATSE + 6-OHDA, ^##p < 0.01, ^###p < 0.001, n = 4 corneas per time point per group). Data represent three independent experiments. b Representative open corneal wounds (revealed by topical fluorescein) and visible wound closure over time post abrasion. c Percent decrease in open wound area over time after corneal wounding of RA or ATSE mice treated with mAb IL-6 or isotype IgG as a control (two-way RM ANOVA, interaction p < 0.0001, Bonferroni’s multiple comparisons test, n = 6 corneas per time point per group). d Number of dividing epithelial cells over time after wounding in RA or ATSE mice treated with mAb IL-6 or isotype IgG control (two-way ANOVA, interaction p < 0.0001, Sidak’s multiple comparisons test, n = 6 corneas per time point per group). e Neutrophil influx into the cornea over time after corneal abrasion in RA or ATSE mice treated with mAb IL-6 or isotype IgG control (two-way ANOVA, interaction p < 0.0001, Sidak’s multiple comparisons test, n = 6 corneas per time point per group). f γδ T cell influx into injured cornea over time after corneal abrasion in RA or ATSE mice treated with mAb IL-6 or isotype IgG control (two-way ANOVA, interaction p < 0.0001, Sidak’s multiple comparisons test, n = 6 corneas per time point per group). *comparison between ATSE + isotype IgG and RA + isotype IgG treatment groups with *p < 0.05, **p < 0.01, and ***p < 0.001; ^# comparison between ATSE + isotype IgG and ATSE + mAb IL-6 groups with ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001