Fig. 8

The α1a-AR antagonist tamsulosin alleviates delayed wound healing and exacerbated inflammation. a Relative expression of adrenergic receptors in sorted CD45⁺GL3⁺ γδ T cells from 20 pooled wounded corneas 18 h after corneal abrasion (Supplementary Figure 5). b Representative open corneal wounds (revealed by topical fluorescein) and visible wound closure over time post wounding. c Percent decrease in open wound area over time after wounding in the RA, ATSE, or ATSE + tamsulosin-treated mice (two-way RM ANOVA, interaction p < 0.0001, Bonferroni’s multiple comparisons test, n = 6 corneas per time point per group). d Epithelial cell division over time after wounding in the RA, ATSE, and ATSE + tamsulosin groups (two-way ANOVA, interaction p < 0.0001, Sidak’s multiple comparisons test, n = 6 corneas per time point per group). e Neutrophil influx into the cornea over time after injury in RA, ATSE, and ATSE + tamsulosin groups (two-way ANOVA, interaction p < 0.0001, Sidak’s multiple comparisons test, n = 6 corneas per time point per group). f γδ T cell influx into the wounded cornea over time after corneal abrasion in the RA, ATSE, and ATSE + tamsulosin groups (two-way ANOVA, interaction p = 0.00513, Sidak’s multiple comparisons test, n = 6 corneas per time point per group). g–i Whole-cornea mRNA expression of NF-kB, IL-6, and IL-17A in injured corneas of the RA, ATSE, and ATSE + topical tamsulosin-treated groups at 6, 12, 18, and 24 h after abrasion (one-way ANOVA, Tukey's post hoc test, RA vs. ATSE, *p < 0.05, **p < 0.01, ***p < 0.001, RA vs. ATSE + tamsulosin; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, n = 6 corneas per group). Symbols denoting statistical significance in c–f: * compares the RA and ATSE groups; ^# compares the ATSE and ATSE + tamsulosin groups. In g–i, symbols compare groups as indicated. *^{, #}p < 0.05; **^{, ##}p < 0.01; ***^{, ###}p < 0.001